ENZYMATIC OXIDATIVE STRESS RESPONSE OF SNAIL (ARCHACHATINA MARGINATA) EXPOSURE TO CHLORPYRIFOS ORGANOPHOSPHATE PESTICIDE TREATED CABBAGE (BRASSICA OLERACEA)

ENZYMATIC OXIDATIVE STRESS RESPONSE OF SNAIL (ARCHACHATINA MARGINATA) EXPOSURE TO CHLORPYRIFOS ORGANOPHOSPHATE PESTICIDE TREATED CABBAGE (BRASSICA OLERACEA)

ENZYMATIC OXIDATIVE STRESS RESPONSE OF SNAIL (ARCHACHATINA
MARGINATA) EXPOSURE TO CHLORPYRIFOS ORGANOPHOSPHATE
PESTICIDE TREATED CABBAGE (BRASSICA OLERACEA)

By

MUSA et al


ABSTRACT
Land snail is a non-target organism when pesticides are applied in the field. The
present study used 60 growers edible giant African land snail Archachatina marginata
of mean weight of 77.00±0.25g fed fresh cabbage Brassica oleracea as bait for the oral
uptake of chlorpyrifos organophosphate pesticide at low environmentally realistic
concentration of 1µM soaked for 1h, under laboratory conditions in plastic snaileries.
The hepatopancrease (digestive gland) was the target organ that was used to analyse
for enzymatic oxidative stress from control and treated groups of chlorpyrifos uptake,
with responses measured at 0, 7, 14, 21 and 28 days of exposure to chemical. The results
showed overall significant (p<0.05) effect in chlorpyrifos treated group, with depressed
mean growth weights indicating effects from reduced feeding. The early activation of
catalase which is the first oxidative defence against chlorpyrifos was influenced by time
of exposure. The digestive enzyme amylase was depressed by chlorpyrifos. The
chlorpyrifos had a significant cyclic effect on lipid. The observed responses can be used
as sensitive biomarkers for the detection and assessment of effects caused by
chlorpyrifos based pesticide.
Keywords: digestive gland, pesticide uptake, non-target organism, hepatopancrease, snail


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