COMPARATIVE STUDY OF HAEMOLYMPH OF THE GIANT AFRICAN LAND SNAILS FOR UREA, CREATINE AND ALKALINE PHOSPHATASE IN ABEOKUTA, SOUTH WESTERN, NIGERIA

COMPARATIVE STUDY OF HAEMOLYMPH OF THE GIANT AFRICAN LAND SNAILS FOR UREA, CREATINE AND ALKALINE PHOSPHATASE IN ABEOKUTA, SOUTH WESTERN, NIGERIA

COMPARATIVE STUDY OF HAEMOLYMPH OF THE GIANT AFRICAN LAND
SNAILS FOR UREA, CREATINE AND ALKALINE PHOSPHATASE IN
ABEOKUTA, SOUTH WESTERN, NIGERIA

By

SODIPE, O.G


ABSTRACT
A comparative study of the haemolymph of two genera of Giant African Land Snails
(Archachatina marginata and Achatina achatina) for urea, creatinine and alkaline
phosphate was investigated. Twenty (20) snails with an average weight of 248±15g forA.
marginata andA. achatina were used for each species. The experiment was laid out in a 2
× 2 factorial arrangement with 20 snails per replicate. The snails were fed with fresh
pawpaw leaves and water for one week to acclimatize after which they were fed a
mixture of chicken layers mash and dried milled pawpaw leaves (1:1; w/w) and water
from the second to fourth week. After 4 weeks, the haemolymph was collected from the
mantle cavity and the foot sinuses using standard method. The haemolymph was
analysed for urea, creatinine and alkaline phosphatase assayed using commercial kits –
(Randox laboratories Ltd., United Kingdom. The result of the study showed that there
was no significant (p>0.05) of the snail genera effect on the haemolymph as well as
interactive effect of snail genera and haemolymph type on haemolymph urea
concentration. The haemolymph creatinine of A. achatina (69.79±3.850 µmol/l) was
significantly higher (p<0.01) than that of the A. marginata (53.66 ± 3.850 µmol/l). There
was a highly significant (p<0.001) difference in haemolymph creatinine between
haemolymph type. Mantle cavity and foot sinuses haemolymph had mean urea of 1.39 ±
0.073 mmol/l and 0.21 ± 0.073 mmol/l; and mean creatinine of 75.91 ± 3.850 µmol/l and
47.54 ± 3.850µmol/l, respectively. There was no significant (p<0.05) interactive effect of
haemolymph creatinine between genera and haemolymph type. The haemolymph
alkaline phosphatase of A. marginata (33.11 ± 1.215 U/L) was significantly higher
(p<0.001) than that of A. achatina (4.97 ± 1.215 U/L). Haemolymph alkaline
phosphatase of mantle cavity haemolymph (25.07 ± 1.215 U/L) was significantly higher
(p<0.001) than that of foot sinuses haemolymph (13.01 ± 1.215 U/L). There was a highly
significantly higher (p<0.001) interactive effect in haemolymph alkaline phosphatase
between genera and haemolymph type. Hence, A. marginata had significantly higher
alkaline phosphatase while A. achatina had significantly higher creatinine. Also, mantle
cavity haemolymph had significantly higher haemolymph urea, creatinine and alkaline
phosphatase than the foot sinuses haemolymph.
Key words: Haemolymph, giant Africa land snail, urea, creatinine, alkaline phosphatase


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